8FSM

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-12-01
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	77.492, 77.492, 37.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.420 - 1.600
R-factor	0.21054
Rwork	0.209
R-free	0.24972
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.008
RMSD bond angle	1.523
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.410	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.098	1.442
Rmeas	0.109	1.746
Rpim	0.046	0.964
Total number of observations	74689	2193
Number of reflections	15223	706
<I/σ(I)>	10.6	1
Completeness [%]	98.6
Redundancy	4.9	3.1
CC(1/2)	0.997	0.406

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection