8FSA
Crystal structure of hen egg white lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 125 |
Detector technology | CCD |
Collection date | 2020-11-03 |
Detector | OXFORD TITAN CCD |
Wavelength(s) | 1.5406 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.404, 77.404, 37.142 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.410 - 1.600 |
R-factor | 0.2215 |
Rwork | 0.219 |
R-free | 0.26713 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qwy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.515 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.400 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.115 | 1.817 |
Rmeas | 0.130 | 2.243 |
Rpim | 0.057 | 1.286 |
Total number of observations | 68827 | 2148 |
Number of reflections | 15256 | 750 |
<I/σ(I)> | 7 | 0.5 |
Completeness [%] | 99.2 | |
Redundancy | 4.5 | 2.9 |
CC(1/2) | 0.993 | 0.289 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |