8FS9

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-11-02
Detector	OXFORD TITAN CCD
Wavelength(s)	1.5406
Spacegroup name	P 43 21 2
Unit cell lengths	78.653, 78.653, 37.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.590 - 1.600
R-factor	0.27198
Rwork	0.270
R-free	0.30522
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qwy
RMSD bond length	0.009
RMSD bond angle	1.559
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.590	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.103	1.026
Rmeas	0.116	1.232
Rpim	0.051	0.667
Total number of observations	74148	2283
Number of reflections	15757	758
<I/σ(I)>	8.7	0.6
Completeness [%]	99.3
Redundancy	4.7	3
CC(1/2)	0.760	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection