8FS9
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2020-11-02 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.653, 78.653, 37.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.590 - 1.600 |
| R-factor | 0.27198 |
| Rwork | 0.270 |
| R-free | 0.30522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qwy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.559 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.590 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.103 | 1.026 |
| Rmeas | 0.116 | 1.232 |
| Rpim | 0.051 | 0.667 |
| Total number of observations | 74148 | 2283 |
| Number of reflections | 15757 | 758 |
| <I/σ(I)> | 8.7 | 0.6 |
| Completeness [%] | 99.3 | |
| Redundancy | 4.7 | 3 |
| CC(1/2) | 0.760 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
