8FRH
Apo structure of D59C mutant of a melibiose transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-07-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 127.930, 127.930, 104.522 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.910 - 3.180 |
R-factor | 0.2626 |
Rwork | 0.260 |
R-free | 0.28630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.511 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3936) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.270 |
High resolution limit [Å] | 3.180 | 3.180 |
Rmerge | 0.149 | 0.149 |
Rmeas | 0.156 | |
Rpim | 0.037 | 0.451 |
Number of reflections | 16869 | 16869 |
<I/σ(I)> | 21.2 | 21.2 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 21.8 | 22.3 |
CC(1/2) | 1.000 | 0.436 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296.15 | 100 mM Tris-HCl, pH 8.5 100 mm NaCl 50 mM CaCL2 32% PEG400 |