8FPW
Crystal structure of tumor related RhoA mutant A161V in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.010, 86.844, 34.337 |
Unit cell angles | 90.00, 93.75, 90.00 |
Refinement procedure
Resolution | 26.900 - 1.400 |
R-factor | 0.1798 |
Rwork | 0.178 |
R-free | 0.20790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.993 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.900 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.108 | 0.379 |
Rmeas | 0.143 | 0.554 |
Rpim | 0.074 | 0.312 |
Number of reflections | 35470 | 4578 |
<I/σ(I)> | 5.2 | 1.8 |
Completeness [%] | 96.5 | 86 |
Redundancy | 3.6 | 2.9 |
CC(1/2) | 0.985 | 0.738 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0 |