8FPR
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2020-09-04 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.568, 77.568, 37.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.430 - 1.600 |
| R-factor | 0.21084 |
| Rwork | 0.209 |
| R-free | 0.25274 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.430 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.106 | 0.974 |
| Rmeas | 0.118 | 1.194 |
| Rpim | 0.049 | 0.680 |
| Total number of observations | 72102 | 2272 |
| Number of reflections | 15432 | 778 |
| <I/σ(I)> | 6.9 | 0.7 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.7 | 2.9 |
| CC(1/2) | 0.995 | 0.589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
