8FPQ
PCSK9 in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-14 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.414, 70.369, 150.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.671 - 2.503 |
| R-factor | 0.1907 |
| Rwork | 0.188 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | FFT |
| Refinement software | PHENIX (1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.000 | 2.512 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.170 | 1.299 |
| Number of reflections | 140840 | 14084 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20.0% Peg-6000, 0.1M Tris pH8.0 |
