8FPC

Conformation 1 of the ligand binding domain (LBDconf1) of GluA2 flip Q isoform of AMPA receptor in complex with gain-of-function TARP gamma-2, with 10mM CaCl2, 150mM NaCl, 1mM MgCl2, 330uM CTZ, and 100mM glutamate (Open-CaNaMg)

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.78
Software	RELION,EPU,CTFFIND,Coot,PHENIX
Symmetry	C2
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION