8FOM

Crystal structure of tRNA^Lys(SUU) bound to UAA codon in the ribosomal P site

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978900
Spacegroup name	P 21 21 21
Unit cell lengths	209.719, 449.479, 617.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.330 - 3.580
R-factor	0.2069
Rwork	0.205
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	153.330	153.330	3.490
High resolution limit [Å]	3.400	15.210	3.400
Rmerge	0.181	0.019	1.282
Rmeas	0.214	0.022	1.510
Total number of observations	2649669
Number of reflections	777325	8770	55511
<I/σ(I)>	7.5	42.84	1.05
Completeness [%]	98.1	92.4	95.2
Redundancy	3.409	3.363	3.429
CC(1/2)	0.996	0.999	0.253

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris-HOAc, pH 7.0, 0.2 M KSCN, 4-4.5% (v/v) PEG 20K, 4.5-5.5% (v/v) PEG 550MME, 10 mM Mg(OAc)2, 0.0028 mM Deoxy BigCHAP