8FN5
Structure of the truncated catalytic domain of Streptococcus mutans GtfD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.625, 126.765, 173.356 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 86.830 - 1.920 |
R-factor | 0.2272 |
Rwork | 0.226 |
R-free | 0.25050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.315 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.830 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.100 | 0.797 |
Rmeas | 0.116 | 0.930 |
Rpim | 0.057 | 0.469 |
Number of reflections | 97783 | 4973 |
<I/σ(I)> | 7.7 | 1.7 |
Completeness [%] | 95.6 | 98.5 |
Redundancy | 3.6 | 3.7 |
CC(1/2) | 0.996 | 0.657 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 295 | 26% PEG 2000MME, 0.1 M Bis-Tris |