8FMS
Complex structure of K210 deletion Troponin complex with neridronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.763, 170.378, 69.709 |
| Unit cell angles | 90.00, 101.74, 90.00 |
Refinement procedure
| Resolution | 43.655 - 3.435 |
| R-factor | 0.2568 |
| Rwork | 0.255 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.450 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.171 | 1.002 |
| Number of reflections | 11980 | 1113 |
| <I/σ(I)> | 10 | 1.5 |
| Completeness [%] | 97.8 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Sodium Acetate, 20% PEG3350 |
