8FMO
Complex structure of K210 deletion Troponin complex with risedronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.379, 169.297, 69.414 |
| Unit cell angles | 90.00, 102.12, 90.00 |
Refinement procedure
| Resolution | 43.391 - 2.612 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.922 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.083 | 0.594 |
| Number of reflections | 26261 | 2421 |
| <I/σ(I)> | 20.3 | 3.1 |
| Completeness [%] | 98.4 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2M Sodium Acetate, 20% PEG3350 |
