8FMJ
Crystal structure of human KRAS in space group R32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-03 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00002 |
Spacegroup name | H 3 2 |
Unit cell lengths | 92.516, 92.516, 117.775 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.450 - 1.330 |
R-factor | 0.1432 |
Rwork | 0.142 |
R-free | 0.16650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.450 | 1.350 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmeas | 0.107 | 1.606 |
Rpim | 0.034 | 0.549 |
Number of reflections | 43647 | 2229 |
<I/σ(I)> | 12.4 | 1.2 |
Completeness [%] | 98.0 | 100 |
Redundancy | 9.8 | 8.4 |
CC(1/2) | 0.999 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris 0.1 M pH 7.8 - 8.0 NaAc 0.2 M PEG3350 30-34% |