8FMF
Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-diAMP ligand (1 tetramer in the AU)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-21 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.92010 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.536, 83.536, 401.960 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 66.990 - 2.100 |
R-factor | 0.1921 |
Rwork | 0.190 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.557 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 133.987 | 2.320 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.251 | 1.961 |
Number of reflections | 64199 | 3211 |
<I/σ(I)> | 8.8 | |
Completeness [%] | 94.8 | |
Redundancy | 12 | |
CC(1/2) | 0.996 | 0.595 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH 5.5-6.0, 0.2 M magnesium chloride, 19-20% PEG3350 |