8FM1
Structure of CBASS Cap5 from Pseudomonas syringae in the absence of a ligand (apo form dimer)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-05 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 159.428, 159.428, 433.875 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 112.730 - 3.160 |
| R-factor | 0.2409 |
| Rwork | 0.240 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc7_3834) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 112.733 | 3.337 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Number of reflections | 85862 | 4294 |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 95.4 | 51.1 |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1 M trisodium citrate, 0.1 M Tris-HCl, pH 8.0 |
