8FLX
De novo designed homotrimer; the fusion product of BGL17 and DHR59
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-02 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.987 |
Spacegroup name | I 2 3 |
Unit cell lengths | 153.477, 153.477, 153.477 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.530 - 4.500 |
R-factor | 0.3064 |
Rwork | 0.302 |
R-free | 0.34410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond angle | 6.603 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.660 |
High resolution limit [Å] | 4.500 | 9.670 | 4.500 |
Rmerge | 1.003 | 0.038 | 0.796 |
Total number of observations | 69242 | ||
Number of reflections | 6944 | 703 | 690 |
<I/σ(I)> | 4.4 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 10 | 10.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 100mM Ammonium citrate tribasic pH 7.0, 10% PEG 3350 |