8FKL
Truncated form of the catalytic domain of Streptococcus mutans GtfB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-11 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.671, 90.862, 92.256 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.740 - 1.480 |
| R-factor | 0.1428 |
| Rwork | 0.141 |
| R-free | 0.17000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.269 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.260 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.081 | 0.627 |
| Rmeas | 0.677 | |
| Rpim | 0.032 | 0.255 |
| Number of reflections | 121563 | 5970 |
| <I/σ(I)> | 13.6 | 2.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7 |
| CC(1/2) | 0.996 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 20% PEG 8000, 0.2 M magnesium chloride, 0.1 M Caps |
