8FK8

Crystal Structure of the Tick Evasin EVA-AAM1001(L39P) Complexed to Human Chemokine CCL7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	P 41 21 2
Unit cell lengths	72.502, 72.502, 65.939
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.780 - 1.960
R-factor	0.2424
Rwork	0.241
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.797
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.780	2.010
High resolution limit [Å]	1.960	1.960
Number of reflections	23718	903
<I/σ(I)>	18.2
Completeness [%]	100.0
Redundancy	25.5
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2 M Ammonium sulfate