8FJF

The three-repeat design H10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-12
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	34.004, 44.287, 78.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.530 - 1.600
R-factor	0.1978
Rwork	0.196
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.049
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.530	1.650
High resolution limit [Å]	1.600	1.600
Rmerge	0.161	1.630
Rpim	0.047	0.472
Number of reflections	30089	1573
<I/σ(I)>	10.22	0.97
Completeness [%]	99.9	99.87
Redundancy	12.9	12.9
CC(1/2)	0.996	0.579

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.12 M D-glucose, 0.12 M D-mannose, 0.12 M D-galactose, 0.12 M L-fucose, 0.12 M D-xylose, 0.12 M N-acetyl-D-glucosamine, 0.0499 M HEPES, 0.0501 M MOPS (acid), 20% v/v PEG 500 MME, and 10% w/v PEG 20,000