8FJE
The five-repeat design E8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.859, 65.763, 87.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.560 - 3.000 |
R-factor | 0.2706 |
Rwork | 0.267 |
R-free | 0.29930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.437 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (dev_4761) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.560 | 3.300 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.203 | 1.240 |
Rpim | 0.060 | 0.346 |
Number of reflections | 5818 | 1428 |
<I/σ(I)> | 7.95 | 2.48 |
Completeness [%] | 98.8 | 99.72 |
Redundancy | 12.7 | 13.6 |
CC(1/2) | 0.993 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M DL-Glutamic acid monohydrate, 0.2M, DL-Alanine, 0.2M Glycine, 0.2M DL-Lysine, 1.0M imidazole, MES monohydrate (acid), 37.5 % v/v of (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350) |