8FIH
Multi-state design of two-state switchable hinge proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.018, 119.757, 38.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.350 - 2.200 |
R-factor | 0.1946 |
Rwork | 0.191 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.559 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.350 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.030 | 1.161 |
Number of reflections | 15520 | 1653 |
<I/σ(I)> | 9.89 | 2.16 |
Completeness [%] | 95.3 | |
Redundancy | 1.4 | 1 |
CC(1/2) | 0.999 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 0.2 M Lithium sulfate, 0.1 M Sodium Phosphate-citrate pH 4.2, 20% PEG 1000 |