8FIA
The structure of fly Teneurin self assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.203, 129.857, 188.158 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.570 - 2.400 |
| R-factor | 0.2162 |
| Rwork | 0.216 |
| R-free | 0.24310 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.570 | 2.940 | 2.330 |
| High resolution limit [Å] | 2.270 | 2.850 | 2.270 |
| Rmerge | 0.157 | 1.717 | 2.537 |
| Rmeas | 0.170 | 1.854 | 2.879 |
| Rpim | 0.066 | 0.693 | 1.334 |
| Number of reflections | 108199 | 4204 | 7876 |
| <I/σ(I)> | 8.6 | 1.4 | 0.5 |
| Completeness [%] | 99.9 | 94 | 99.8 |
| Redundancy | 6.6 | 6.9 | 4.5 |
| CC(1/2) | 0.996 | 0.535 | 0.257 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium Acetate, 20% PEG 3350 | |
| 2 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M Ammonium Sulfate, 16% PEG 3350 |
