8FIA
The structure of fly Teneurin self assembly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 95.203, 129.857, 188.158 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.570 - 2.400 |
R-factor | 0.2162 |
Rwork | 0.216 |
R-free | 0.24310 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.256 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.570 | 2.940 | 2.330 |
High resolution limit [Å] | 2.270 | 2.850 | 2.270 |
Rmerge | 0.157 | 1.717 | 2.537 |
Rmeas | 0.170 | 1.854 | 2.879 |
Rpim | 0.066 | 0.693 | 1.334 |
Number of reflections | 108199 | 4204 | 7876 |
<I/σ(I)> | 8.6 | 1.4 | 0.5 |
Completeness [%] | 99.9 | 94 | 99.8 |
Redundancy | 6.6 | 6.9 | 4.5 |
CC(1/2) | 0.996 | 0.535 | 0.257 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Ammonium Acetate, 20% PEG 3350 | |
2 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5 M Ammonium Sulfate, 16% PEG 3350 |