8FI7
Structure of Lettuce C20T bound to DFHO
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-06-24 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.105 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.571, 43.104, 117.641 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.770 - 2.900 |
R-factor | 0.2471 |
Rwork | 0.244 |
R-free | 0.28900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8fhv |
RMSD bond length | 0.005 |
RMSD bond angle | 0.679 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.470 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.164 | 0.442 |
Number of reflections | 3374 | 325 |
<I/σ(I)> | 22.8 | 2.5 |
Completeness [%] | 98.6 | |
Redundancy | 9.15 | |
CC(1/2) | 0.996 | 0.958 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.05 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% (v/v) polyethylene glycol monoethyl ether 550 |