8FI0

Structure of Lettuce aptamer bound to DFAME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	24.876, 43.022, 119.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.810 - 3.000
R-factor	0.2356
Rwork	0.228
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8fhv
RMSD bond length	0.004
RMSD bond angle	0.662
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.810	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.070	0.269
Number of reflections	3164	300
<I/σ(I)>	20	3.1
Completeness [%]	99.9
Redundancy	11.46
CC(1/2)	0.994	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	0.05 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% (v/v) polyethylene glycol monoethyl ether 550