8FHL
Structure of pyruvate dehydrogenase phosphatase regulatory subunit neoepitope presented by H2-Dd
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-23 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 47.091, 86.359, 211.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.343 - 2.194 |
Rwork | 0.210 |
R-free | 0.24790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8d5f |
RMSD bond length | 0.006 |
RMSD bond angle | 1.392 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.343 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.187 | |
Rmeas | 0.195 | |
Rpim | 0.055 | |
Number of reflections | 29908 | 2215 |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.8 | |
Redundancy | 13.1 | |
CC(1/2) | 0.997 | 0.367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2 M sodium isothiocyanate, 20% PEG4000 |