8FHJ
Crystal structure of a FAD monooxygenease from Methylocystis sp. Strain SB2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.347, 96.765, 135.206 |
Unit cell angles | 90.00, 116.41, 90.00 |
Refinement procedure
Resolution | 59.950 - 2.610 |
R-factor | 0.2062 |
Rwork | 0.204 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ihg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 121.100 | 2.750 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.126 | 1.692 |
Rmeas | 0.149 | 1.986 |
Rpim | 0.079 | 1.033 |
Total number of observations | 184364 | 27645 |
Number of reflections | 52358 | 7601 |
<I/σ(I)> | 6.4 | 0.8 |
Completeness [%] | 99.8 | |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.996 | 0.461 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 20% PEG3350, 0.1 M Bis-Tris propane pH 6.5, 0.2 M Sodium bromide |