8FHC

Protein 41 with aldehyde deformylating oxidase activity from Gamma proteobacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	48.296, 85.711, 112.558
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.443 - 2.097
Rwork	0.195
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.356
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.443	2.160
High resolution limit [Å]	2.097	2.100
Rmerge	0.107	0.579
Rmeas	0.113	0.614
Rpim	0.035	0.198
Number of reflections	28065	2195
<I/σ(I)>	16
Completeness [%]	99.6
Redundancy	9.9	9.2
CC(1/2)	0.998	0.926

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	27% PEG 3350, 200 mM NaCl, 1.5 mM cholalic acid sodium salt, 100 mM imidazole