8FFT
Structure of GntC, a PLP-dependent enzyme catalyzing L-enduracididine biosynthesis from (S)-4-hydroxy-L-arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99992 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.855, 158.159, 73.231 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 73.230 - 2.100 |
R-factor | 0.2211 |
Rwork | 0.220 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3op7 |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | AutoProcess |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.230 | 2.106 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 75093 | 778 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 93.1 | |
Redundancy | 5.2 | |
CC(1/2) | 0.573 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283 | 19% (w/v) PEG 3350, 0.50 M MgCl2, 0.10 M Tris pH 8.5, and 1 mM PLP |