8FFR

Revised structure of the rabies virus nucleoprotein-RNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-25
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.9394
Spacegroup name	P 21 21 2
Unit cell lengths	270.430, 281.000, 236.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.750 - 3.490
R-factor	0.2253
Rwork	0.224
R-free	0.25520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.346
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.750	3.630
High resolution limit [Å]	3.490	3.490
Rmerge	0.096
Number of reflections	226498	21091
<I/σ(I)>	12.15	3.36
Completeness [%]	99.9
Redundancy	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	60% TACSIMATE, 10% L-CYSTEIN, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K