8FF9

Crystal structure of Apo Dps protein (PA0962) from Pseudomonas aeruginosa (orthorhombic form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	291
Detector technology	PIXEL
Collection date	2020-08-01
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	129.541, 129.820, 156.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.430 - 1.700
R-factor	0.1473
Rwork	0.146
R-free	0.16950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3959)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.105	1.159
Rmeas	0.114	1.252
Rpim	0.043	0.469
Total number of observations	1964310	98554
Number of reflections	288462	14185
<I/σ(I)>	10.8	1.8
Completeness [%]	100.0
Redundancy	6.8	6.9
CC(1/2)	0.997	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1 M (NH4)2SO4, 1M KCl, 100 mM HEPES