8FF9
Crystal structure of Apo Dps protein (PA0962) from Pseudomonas aeruginosa (orthorhombic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2020-08-01 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 129.541, 129.820, 156.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.430 - 1.700 |
R-factor | 0.1473 |
Rwork | 0.146 |
R-free | 0.16950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.946 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3959) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.430 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.105 | 1.159 |
Rmeas | 0.114 | 1.252 |
Rpim | 0.043 | 0.469 |
Total number of observations | 1964310 | 98554 |
Number of reflections | 288462 | 14185 |
<I/σ(I)> | 10.8 | 1.8 |
Completeness [%] | 100.0 | |
Redundancy | 6.8 | 6.9 |
CC(1/2) | 0.997 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 1 M (NH4)2SO4, 1M KCl, 100 mM HEPES |