8FEQ
16mer self-complementary duplex RNA with two separated s(2)U:s(2)U pairs
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 2.0.1 |
Synchrotron site | ALS |
Beamline | 2.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.037690 |
Spacegroup name | H 3 2 |
Unit cell lengths | 41.261, 41.261, 124.239 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.993 - 1.500 |
Rwork | 0.177 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 2.577 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.077 | 0.235 |
Rmeas | 0.082 | 0.249 |
Rpim | 0.029 | 0.081 |
Number of reflections | 6853 | 352 |
<I/σ(I)> | 14.2 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.7 | 9 |
CC(1/2) | 0.998 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.075 M Sodium chloride, 0.002 M Calcium chloride dihydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0, 30% w/v 1,6 Hexanediol, 0.0005 M Spermine |