8FEO
16mer self-complementary duplex RNA with two separated native U:U pairs
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033175 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.503, 41.503, 125.299 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.796 - 1.520 |
| Rwork | 0.198 |
| R-free | 0.23930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.559 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.800 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.075 | 0.234 |
| Rmeas | 0.081 | 0.253 |
| Rpim | 0.029 | 0.093 |
| Number of reflections | 6587 | 322 |
| <I/σ(I)> | 36.4 | 3 |
| Completeness [%] | 98.3 | 99.1 |
| Redundancy | 8.4 | 7 |
| CC(1/2) | 0.998 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate pH 7.0, 20% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride |
