8FDS

Ankyrin domain of SKD3 isoform 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	60.440, 60.440, 180.313
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.340 - 1.650
R-factor	0.2002
Rwork	0.199
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8deh
RMSD bond length	0.007
RMSD bond angle	0.863
Data reduction software	DIALS (3.6.2)
Data scaling software	DIALS (3.6.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.340	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.101	0.532
Rmeas	0.104
Rpim	0.024	0.125
Number of reflections	24410	1202
<I/σ(I)>	9	0.4
Completeness [%]	100.0	100
Redundancy	18.3	19.1
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	287	4 M potassium formate, 0.1 M Bis-Tris propane, pH 9.0, 2% w/v PEG2000 MME