8FD1
Crystal structure of photoactivated rhodopsin in complex with a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.289, 120.289, 227.577 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.360 - 4.250 |
| R-factor | 0.28 |
| Rwork | 0.279 |
| R-free | 0.29520 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.045 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.510 |
| High resolution limit [Å] | 4.240 | 4.250 |
| Rmerge | 0.058 | 3.550 |
| Number of reflections | 13967 | 2196 |
| <I/σ(I)> | 14.28 | 0.72 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.1 | |
| CC(1/2) | 1.000 | 0.264 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277.15 | 25.5% PEG 600 0.1 M Tricine pH 7.8 5 mM EDTA 5 mM beta mercaptoethanol 50 mM Cyglu-4 |
