8FC2

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and azithromycin at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-01
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	209.900, 450.730, 622.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	174.040 - 2.500
R-factor	0.2093
Rwork	0.207
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhx
RMSD bond length	0.004
RMSD bond angle	0.881
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	174.040	174.040	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.185	0.040	2.076
Rmeas	0.200	0.043	2.241
Total number of observations	13772109
Number of reflections	2002056	23374	147365
<I/σ(I)>	8.53	33.8	1
Completeness [%]	99.8	98.9	99.7
Redundancy	6.879	7.041	6.992
CC(1/2)	0.995	0.998	0.190

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME