8FC1
Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.50A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 208.570, 448.580, 617.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 151.650 - 2.500 |
R-factor | 0.2078 |
Rwork | 0.206 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6xhx |
RMSD bond length | 0.004 |
RMSD bond angle | 0.883 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 151.650 | 151.650 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.169 | 0.047 | 2.402 |
Rmeas | 0.183 | 0.051 | 2.586 |
Total number of observations | 13707917 | ||
Number of reflections | 1965632 | 23144 | 144933 |
<I/σ(I)> | 9.13 | 34.06 | 0.86 |
Completeness [%] | 99.9 | 99.8 | 100 |
Redundancy | 6.974 | 6.989 | 7.207 |
CC(1/2) | 0.995 | 0.995 | 0.203 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |