8FC0
Crystal structure of a metagenome-derived GH5 endo-beta-1,4-glucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.559, 97.823, 100.198 |
| Unit cell angles | 66.05, 69.68, 70.02 |
Refinement procedure
| Resolution | 29.000 - 2.100 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | Aimless (1.10.28) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.143 | 1.615 |
| Rmeas | 0.170 | 2.010 |
| Rpim | 0.091 | 1.169 |
| Total number of observations | 676536 | 27537 |
| Number of reflections | 196668 | 9457 |
| <I/σ(I)> | 5.4 | 0.5 |
| Completeness [%] | 94.3 | |
| Redundancy | 3.4 | 2.9 |
| CC(1/2) | 0.991 | 0.217 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 20% PEG3350, 0.2 M sodium bromide, and 0.1 M de BIS-TRIS propane, pH 6,5 |
