8FBY
Crystal structure of PLVAP CC1 determined by sulfur SAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.7712 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.587, 86.232, 181.647 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.820 - 2.400 |
R-factor | 0.225 |
Rwork | 0.223 |
R-free | 0.26100 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.170 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.830 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.122 | 0.345 |
Rmeas | 0.123 | 0.350 |
Rpim | 0.016 | 0.056 |
Total number of observations | 1374893 | 59112 |
Number of reflections | 22760 | 1564 |
<I/σ(I)> | 29.6 | 10.6 |
Completeness [%] | 99.7 | |
Redundancy | 60.4 | 37.8 |
CC(1/2) | 0.998 | 0.991 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium malonate, pH 5.0 and 20% PEG3350 |