8FBT
Crystal structure of Cryptosporidium parvum N-myristoyltransferase with bound myristoyl-CoA
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-04-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.999989 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.433, 105.338, 71.120 |
Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
Resolution | 49.830 - 2.200 |
R-factor | 0.1663 |
Rwork | 0.164 |
R-free | 0.21720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.177 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmeas | 0.083 | 0.703 |
Rpim | 0.043 | 0.359 |
Number of reflections | 45534 | 2260 |
<I/σ(I)> | 19.11 | 2.06 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.996 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | CrpaA.18219.a.A42.PS38366 32 mg/mL was incubated with final concentrations of 0.7 mM Myristoyl-CoA at 4C for 30 min, then 0.6 microliter was mixed with 1:1 BisTris-HCl, pH 5.5, 25% PEG 3350. Cryo solution contained crystallant plus 27% PEG-400 |