8FBN
Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-06-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.997, 170.813, 72.148 |
Unit cell angles | 90.00, 117.83, 90.00 |
Refinement procedure
Resolution | 42.700 - 3.040 |
R-factor | 0.2587 |
Rwork | 0.255 |
R-free | 0.30020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.371 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.700 | 3.110 |
High resolution limit [Å] | 3.040 | 3.040 |
Rmerge | 0.151 | 1.631 |
Number of reflections | 29424 | 4770 |
<I/σ(I)> | 4.8 | 0.8 |
Completeness [%] | 99.2 | 99.9 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.988 | 0.293 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M Tris, pH 7.0, 20% w/v PEG2000 MME |