8FBJ
Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-20 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | H 3 |
Unit cell lengths | 89.917, 89.917, 124.897 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.630 - 3.250 |
R-factor | 0.2419 |
Rwork | 0.240 |
R-free | 0.28490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.336 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.990 | 3.510 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.119 | 0.261 |
Number of reflections | 5943 | 11095 |
<I/σ(I)> | 10.2 | 5.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.6 | 8.6 |
CC(1/2) | 0.998 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M magnesium chloride, 0.1 M Tris, pH 7.0, 10% w/v PEG8000 |