8FBA

Crystal structure of Ky315 Fab in complex with circumsporozoite protein DND peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920088
Spacegroup name	P 1 21 1
Unit cell lengths	67.348, 65.245, 128.690
Unit cell angles	90.00, 97.25, 90.00

Refinement procedure

Resolution	39.370 - 1.960
R-factor	0.1788
Rwork	0.178
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 6ULF
RMSD bond length	0.005
RMSD bond angle	0.758
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.990
High resolution limit [Å]	1.960	1.960
Number of reflections	79772	3529
<I/σ(I)>	9.6
Completeness [%]	100.0
Redundancy	7
CC(1/2)	0.996	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M magnesium formate pH 5.9, 20% PEG3350