8FB8
Crystal structure of Ky15.10-Y100EK Fab in complex with circumsporozoite protein KQPA peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033167 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.385, 47.721, 85.012 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 37.340 - 1.690 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.18430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.710 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 51242 | 1783 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 98.9 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.658 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES pH 7.5, 20% PEG8000 |
