8FB6

Crystal structure of Ky15.10 Fab in complex with circumsporozoite protein KQPA peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	52.606, 45.668, 83.111
Unit cell angles	90.00, 97.64, 90.00

Refinement procedure

Resolution	34.640 - 2.150
R-factor	0.1914
Rwork	0.189
R-free	0.23630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.180
High resolution limit [Å]	2.150	2.150
Number of reflections	21555	880
<I/σ(I)>	9.7
Completeness [%]	99.9
Redundancy	6.5
CC(1/2)	0.994	0.734

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M sodium acetate pH 4.5, 40% 1,2-propanediol