8FB3
PreQ1-1 (type-1) riboswitch with stacked metabolites and a C10-G34 base pair in the expression platform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.867, 67.612, 50.589 |
| Unit cell angles | 90.00, 100.53, 90.00 |
Refinement procedure
| Resolution | 35.160 - 3.000 |
| R-factor | 0.2286 |
| Rwork | 0.225 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.726 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX ((1.20.1_4487)) |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.200 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rpim | 0.051 | 0.339 |
| Number of reflections | 5588 | 871 |
| <I/σ(I)> | 7.6 | 1.4 |
| Completeness [%] | 99.0 | 92.2 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG 8000, 0.2 M ammonium acetate, 0.01 M magnesium acetate tetrahydrate, and 0.05 M sodium cacodylate pH 6.5 |
