8FAW
Structure of Hemagglutinin from Influenza A/Victoria/22/2020 in complex with LSTC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-04-25 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.349, 100.349, 395.503 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.570 - 2.160 |
| R-factor | 0.20085 |
| Rwork | 0.199 |
| R-free | 0.23479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.505 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.880 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.085 | 1.099 |
| Number of reflections | 40001 | 4880 |
| <I/σ(I)> | 26.6 | |
| Completeness [%] | 96.1 | |
| Redundancy | 21.3 | |
| CC(1/2) | 1.000 | 0.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291.15 | 50% PEG-200, 0.1M citrate pH 5.5, final pH 6.9 |
