8FAT
Crystal structure of Ky224 Fab in complex with circumsporozoite protein NPDP peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.515, 115.262, 120.385 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.900 - 2.950 |
R-factor | 0.2265 |
Rwork | 0.224 |
R-free | 0.27430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6o25 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.509 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 19561 | 929 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 99.8 | |
Redundancy | 10.7 | |
CC(1/2) | 0.981 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 |