8FAN
Crystal structure of Ky15.1 Fab in complex with circumsporozoite protein KQPA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033170 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 181.681, 181.681, 202.648 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.340 - 2.900 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.25010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u6v |
RMSD bond length | 0.002 |
RMSD bond angle | 0.546 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 43443 | 2146 |
<I/σ(I)> | 7.7 | |
Completeness [%] | 98.1 | |
Redundancy | 10 | |
CC(1/2) | 0.980 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.4 M ammonium sulfate, 0.1 M Tris pH 8.5 |