8FAA

Crystal structure of Xanthomonas campestris GH35 beta-galactosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-25
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	128.271, 162.991, 235.478
Unit cell angles	90.00, 92.83, 90.00

Refinement procedure

Resolution	49.100 - 2.500
R-factor	0.2131
Rwork	0.211
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.846
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	332061	15162
<I/σ(I)>	7.4
Completeness [%]	99.1
Redundancy	4.2
CC(1/2)	0.980	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	calcium acetate 0.2M PEG3350 20%