8FA9
Crystal structure of Ky15.5 Fab in complex with circumsporozoite protein NPDP peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03319 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 99.852, 87.786, 71.587 |
Unit cell angles | 90.00, 119.28, 90.00 |
Refinement procedure
Resolution | 28.140 - 2.450 |
R-factor | 0.188 |
Rwork | 0.187 |
R-free | 0.21220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.597 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.490 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 18410 | 894 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 92.1 | |
Redundancy | 2.5 | |
CC(1/2) | 0.990 | 0.571 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG3350, 0.2 M tri-lithium citrate |